東南大學采用我司蛋白G磁珠偶聯(lián)抗體捕獲細菌進行檢測發(fā)表論文
東南大學采用我司蛋白G磁珠偶聯(lián)抗體捕獲細菌進行檢測發(fā)表論文
Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection
基于Ag陽極溶解和陰極氧還原生物催化金黃色葡萄球菌檢測的時間傳感平臺
Abstract
An Ag@C hybrid bipolar electrode (BPE) sensing platform has been established for the temporal detection of Staphylococcus aureus (S. aureus) in food. Combining the advantages of anodic dissolution of Ag and cathodic biocatalysis of oxygen (O2) reduction, this strategy showed an ultralow detection limit down to 10 CFU mL?1. As the formation of Ag@C completely quenched the electrochemiluminescence (ECL) emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Meanwhile, S. aureus catalyzed the electrochemical reduction of O2 at the cathode, reducing the overpotential for cathodic O2 reduction and thus increasing the rate of anodic electron loss, facilitating Ag dissolution and restoring the ECL emission of luminol. When a constant potential was applied, through monitoring the ECL recovery time before and after the incubation of S. aureus on the cathode, S. aureus could be quantified due to the slight difference of the conductivity.
摘要
建立了Ag@C混合雙極電極（BPE）傳感平臺，用于食品中金黃色葡萄球菌（S. aureus）的時間檢測。結合 Ag 的陽極溶解和氧 (O2) 還原的陰極生物催化的優(yōu)勢，該策略顯示出低至 10 CFU mL-1 的檢測限。由于 Ag@C 的形成完全淬滅了魯米諾的電化學發(fā)光 (ECL) 發(fā)射，因此 ECL 發(fā)射恢復反映了陽極溶解的程度。同時，金黃色葡萄球菌在陰極催化了O2的電化學還原，降低了陰極O2還原的過電位，從而增加了陽極電子損失率，促進了Ag的溶解，恢復了魯米諾的ECL發(fā)射。當施加恒定電位時，通過監(jiān)測金黃色葡萄球菌在陰極上孵育前后的ECL恢復時間，由于電導率的微小差異，可以對金黃色葡萄球菌進行量化。
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