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客戶(hù)采用我司G-ProA蛋白A磁珠偶聯(lián)抗體在Current Microbiology發(fā)表論文

2023-9-24 22:51:57點(diǎn)擊：


Zheng, H., et al. Establishment of a Fast Diagnostic Method for Sepsis Pathogens Based on M1 Bead Enrichment. Curr Microbiol 80, 166 (2023). https://doi.org/10.1007/s00284-023-03280-6 (G-ProA蛋白A磁珠偶聯(lián)抗體)


Establishment of a Fast Diagnostic Method for Sepsis Pathogens Based on M1 Bead Enrichment

基于M1磁珠富集的膿毒癥病原菌快速診斷方法的建立

Hao Zheng1  · Xiaoli Chen1  · Wenge Li 1  · Jinxing Lu1  · Xiaoping Chen1

Received: 22 May 2022 / Accepted: 21 March 2023 / Published online: 6 April 2023 © The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2023


Abstract
Blood culture-based sepsis diagnostic methods usually cannot obtain positive results in a timely manner. Molecular diagnostic methods, such as real-time PCR without blood culture, would be more time-saving and suitable for pathogenic diagnosis of sepsis, while their sensitivities have always been unsatisfactory for the usually low concentration of pathogens in the blood of sepsis patients. In this study, we established a fast diagnostic method using magnetic beads coated with human recombined mannose-binding lectin that makes it possible to concentrate pathogens from human plasma that have low concentrations of pathogens. With subsequent microculture (MC) and real-time PCR, this method allowed the detection of 1–10 CFUs/ ml of Staphylococcus aureus, Group A Streptococcus, Escherichia coli, Pseudomonas aeruginosa, Candida tropicalis, or
C. albicans from human plasma within 9.5 h, which was 21–80 h earlier than blood culture. The combination of pathogen enrichment and MC made the detection of sepsis-causing pathogens more time-saving and more sensitive than blood culture or real-time PCR alone.


摘要：
基于血培養(yǎng)的膿毒癥診斷方法通常不能及時(shí)獲得陽(yáng)性結(jié)果。分子診斷方法，如無(wú)血培養(yǎng)的實(shí)時(shí)熒光定量PCR，更省時(shí)，適合于膿毒癥的病原診斷，而對(duì)于膿毒癥患者血液中通常低濃度的病原體，其敏感性一直不盡如人意。在這項(xiàng)研究中，我們建立了一種快速診斷方法，使用涂有人重組甘露糖結(jié)合凝集素的磁珠，該方法可以從具有低濃度病原體的人血漿中濃縮病原體。通過(guò)隨后的顯微培養(yǎng) （MC） 和實(shí)時(shí)熒光定量 PCR，該方法可檢測(cè) 1-10 CFU/ml 金黃色葡萄球菌、A 組鏈球菌、大腸桿菌、銅綠假單胞菌、熱帶念珠菌或
9.5小時(shí)內(nèi)從人血漿中取出白色念珠菌，比血培養(yǎng)早21-80小時(shí)。病原體富集和MC的結(jié)合使得檢測(cè)引起膿毒癥的病原體比單獨(dú)的血培養(yǎng)或?qū)崟r(shí)熒光定量PCR更省時(shí)，更靈敏。


更多蛋白A磁珠信息請(qǐng)參考 蛋白A磁珠|Protein A磁珠|Protein A magnetic bead|免疫沉淀|抗體純化-生物磁珠專(zhuān)家 (tools.7z1ocr.cn)

更多蛋白G磁珠信息請(qǐng)參考 蛋白G磁珠|ProteinG磁珠|Protein G magnetic bead-生物磁珠專(zhuān)家 (tools.7z1ocr.cn)