客戶采用我司不同修飾硅磁珠研究核酸提取機(jī)理在《Separation and Purification Technology》發(fā)表SCI論文
客戶采用我司不同修飾硅磁珠，研究了磁珠大小、磁珠表面基團(tuán)在動(dòng)物組織樣本基因組提取過(guò)程中的影響，為核酸提取磁珠的選擇使用提供理論支撐。
Peipei Li, Menghang Li, Fan Zhang, Mengyuan Wu, Xiaobin Jiang, Bo Ye, Zhenjun Zhao, Dongmei Yue, Qi Fan, Haixia Chen, High-efficient nucleic acid separation from animal tissue samples via surface modified magnetic nanoparticles,Separation and Purification Technology,Volume 262,2021,118348,
Abstract
High efficiency nucleic acid (NA) separation via magnetic nanoparticles (MNPs) is a promising technology for bioengineering, healthcare, etc. Aimed to enhance the selection of MNPs and illustrate the separation mechanism for effective isolation of NAs from complex animal samples, this study fabricated hydroxy-, amino- and carboxyl-functionalized MNPs (MNPs-OH, MNPs-NH2 and MNPs-COOH) with different particle sizes to investigate the hybrid NA separation performance from animal tissue samples. Molecular dynamics (MD) simulation was introduced to predict the affinity action of MNPs with NA and typical impurities. The details of the kinetic separation behavior and the NA separation performance were then illustrated. MD simulation results showed MNPs-NH2 had a stronger affinity with NA, and in most experimental separation systems, NA separation yield via three kinds of MNPs showed a following order of MNPs-NH2 > MNPs-OH > MNPs-COOH. MNPs of different sizes had significantly different sedimentation and dispersion features, leading to diverse separation results. Combining with the proper lysis system, an optimized NA separation method at room temperature with high-throughput and low contamination risk was developed as a promising approach for large-scale samples extracted NA simultaneously. This work could provide a theoretical guidance for the fabrication strategy of advanced MNPs and the enhancement of NA separation from complex samples.
摘要
利用磁性納米粒子（MNPs）高效分離核酸是生物工程、醫(yī)療保健等領(lǐng)域的一項(xiàng)很有前途的技術(shù)。為了提高M(jìn)NPs的選擇性，闡明從復(fù)雜動(dòng)物樣品中有效分離核酸的分離機(jī)理，本研究制備了羥基-，研究了不同粒徑的氨基和羧基功能化MNPs（MNPs-OH、MNPs-NH2和MNPs-COOH）從動(dòng)物組織樣品中分離雜交NA的性能。采用分子動(dòng)力學(xué)模擬方法預(yù)測(cè)了MNPs與NA及典型雜質(zhì)的親和作用。詳細(xì)說(shuō)明了動(dòng)力學(xué)分離行為和鈉分離性能。MD模擬結(jié)果表明，MNPs-NH2對(duì)NA有較強(qiáng)的親和性，在大多數(shù)實(shí)驗(yàn)分離體系中，三種MNPs對(duì)NA的分離率依次為MNPs-NH2>MNPs-OH>MNPs-COOH。不同粒徑的MNPs具有明顯不同的沉降和分散特性，導(dǎo)致分離效果不同。結(jié)合合適的裂解體系，建立了一種高通量、低污染風(fēng)險(xiǎn)的室溫鈉分離方法，為大規(guī)模樣品同時(shí)提取鈉提供了一種新的途徑。這項(xiàng)工作為先進(jìn)MNPs的制備策略和提高復(fù)雜樣品中NA的分離提供了理論指導(dǎo)。
更多核酸提取磁珠請(qǐng)參考： http://www.tools.7z1ocr.cn/Product/267584288.html
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